Direct PCR amplification and sequencing of specimens' DNA from preservative ethanol
نویسندگان
چکیده
منابع مشابه
Direct PCR amplification and sequencing of specimens' DNA from preservative ethanol.
DNA extraction is the first step in many molecular biology protocols. However, we hypothesized that DNA from a preserved specimen can leak into its preservative medium, allowing the medium itself to be directly PCR-amplified. We successfully tested this idea on mescal-the alcoholic beverage famous for the "worm" (a caterpillar) that is placed in the bottle of many brands-and indeed obtained amp...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2010
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113362